Assessment of Cell Viability of Fresh Osteochondral Allografts in N-Acetylcysteine-Enriched Medium
نویسندگان
چکیده
منابع مشابه
Human articular cartilage storage in cell culture medium: guidelines for storage of fresh osteochondral allografts.
The viability of transplanted articular cartilage is one of the determinants of outcome following the transplantation of osteochondral allografts. Disappointing results from cryopreservation have led to the practice of fresh transplantation of articular segments, especially for posttraumatic defects. To date, no studies have demonstrated in vitro viability rates for refrigerated human cartilage...
متن کاملChondrocyte viability in fresh and frozen large human osteochondral allografts: effect of cryoprotective agents.
Chondrocyte survival is a major goal for the effective storage and clinical performance of human osteochondral allografts. The majority of animal and human cryopreservation studies conducted so far have been performed in small osteochondral cylinders. Using human tibial plateaus as a model for large osteochondral pieces, this work sought to evaluate the cryoprotective efficiency of glycerol and...
متن کاملComparison of fresh osteochondral autografts and allografts: a canine model.
BACKGROUND Osteochondral autografts and allografts have been widely used in the treatment of isolated grade IV articular cartilage lesions of the knee. However, the authors are not aware of any study that has prospectively compared fresh osteochondral autografts to fresh allografts with regard to imaging, biomechanical testing, and histology. HYPOTHESIS The imaging, biomechanical properties, ...
متن کاملChondrocyte viability in press-fit cryopreserved osteochondral allografts.
The viability of chondrocytes in press-fit glycerol-preserved osteochondral allografts was compared to that in fresh autografts, after transplantation into load-bearing and non-load-bearing sites in mature sheep stifle joints. We used macroscopic grading, tonometer pen indentation testing, histology, sulfate uptake and viability as determined by confocal-microscopy to assess cartilage condition...
متن کاملIntermediate outcomes of fresh talar osteochondral allografts for treatment of large osteochondral lesions of the talus.
BACKGROUND Large osteochondral defects of the talus present a treatment challenge. Fresh osteochondral allograft transplantation can be used for large lesions without the donor-site morbidity associated with other procedures such as autologous chondrocyte implantation or osteochondral autograft transfer. The goal of this study was to prospectively evaluate the intermediate outcomes of fresh ost...
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ژورنال
عنوان ژورنال: CARTILAGE
سال: 2018
ISSN: 1947-6035,1947-6043
DOI: 10.1177/1947603518786547